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Fluorescence intensity and lifetime distribution analysis: Toward higher accuracy in fluorescence fluctuation spectroscopy
Fluorescence fluctuation methods such as fluorescence correlation spectroscopy and fluorescence intensity distribution analysis (FIDA) have proven to be versatile tools for studying molecular interactions...
PublicationsRapid analysis of Forster resonance energy transfer by two-color global fluorescence correlation spectroscopy: Trypsin proteinase reaction
In this study we introduce the combination of two-color global fluorescence correlation spectroscopy (2CG-FCS) and Forster resonance energy transfer (FRET) as a very powerful combination for monitoring...
PublicationsTwo-color far-field fluorescence nanoscopy
We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and...
PublicationsFluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the...
PublicationsGeneration of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy
Reversibly switchable fluorescent proteins (RSFPs) are GFP-like proteins that may be repeatedly switched by irradiation with light from a fluorescent to a nonfluorescent state, and vice versa. They can...
PublicationsCharacterization of Horizontal Lipid Bilayers as a Model System to Study Lipid Phase Separation
Artificial lipid membranes are widely used as a model system to study single ion channel activity using electrophysiological techniques. In this study, we characterize the properties of the artificial...
PublicationsMulticolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength
Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction...
PublicationsSTED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells
Details about molecular membrane dynamics in living cells, such as lipid-protein interactions, are often hidden from the observer because of the limited spatial resolution of conventional far-field optical...
PublicationsMembrane Orientation and Lateral Diffusion of BODIPY-Cholesterol as a Function of Probe Structure
Cholesterol tagged with the BODIPY fluorophore via the central difluoroboron moiety of the dye (B-Chol) is a promising probe for studying intracellular cholesterol dynamics. We synthesized a new BODIPY-cholesterol...
PublicationsReorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy
Myelin is a multilayered membrane that ensheathes axonal fibers in the vertebrate nervous system, allowing fast propagation of nerve action potentials. It contains densely packed lipids, lacks an actin-based...
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