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Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy
The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids...
PublicationsModulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles
The transfer of material from phospholipid-coated microbubbles to cell membranes has been hypothesized to play a role in ultrasound-mediated drug delivery. In this study, we employed quantitative fluorescence...
PublicationsCritical importance of appropriate fixation conditions for faithful imaging of receptor microclusters
Receptor clustering is known to trigger signalling events that contribute to critical changes in cellular functions. Faithful imaging of such clusters by means of fluorescence microscopy relies on the...
PublicationsFoCuS-point: software for STED fluorescence correlation and time-gated single photon counting
Motivation: Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility and how mobility relates to molecular interaction dynamics and bioactivity in living cells. The...
PublicationsWhy do peroxisomes associate with the cytoskeleton?
Attachment of peroxisomes to cytoskeleton and movement along microtubular filaments and actin cables are essential and highly regulated processes enabling metabolic efficiency, biogenesis, maintenance...
PublicationsRegulation of peroxisomal matrix protein import by ubiquitination
Peroxisomes are organelles that play an important role in many cellular tasks. The functionality of peroxisomes depends on the proper import of their matrix proteins. Peroxisomal matrix proteins are imported...
PublicationsPartitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes
Several simplified membrane models featuring coexisting liquid disordered (Ld) and ordered (Lo) lipid phases have been developed to mimic the heterogeneous organization of cellular membranes, and thus,...
PublicationsClosing the gap: The approach of optical and computational microscopy to uncover biomembrane organization
Biological membranes are complex composites of lipids, proteins and sugars, which catalyze a myriad of vital cellular reactions in a spatiotemporal tightly controlled manner. Our understanding of the organization...
Publications1.8 angstrom bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
RSFPs (reversibly switchable fluorescent proteins) may be repeatedly converted between a fluorescent and a non-fluorescent state by irradiation and have attracted widespread interest for many new applications....
PublicationsDetection and characterization of single molecules in aqueous solution
Using a confocal microscope with a single-photon avalanche photodiode as detector, we studied photon bursts of single Rhodamine 6G (R6G) and Rhodamin B-zwitterion (RE) molecules in aqueous solution by...
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