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Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins
Membrane-associated events during peroxisomal protein import processes play an essential role in peroxisome functionality. Many details of these processes are not known due to missing spatial resolution...
PublicationsPhase Partitioning of GM1 and Its Bodipy-Labeled Analog Determine Their Different Binding to Cholera Toxin
Driven by interactions between lipids and proteins, biological membranes display lateral heterogeneity that manifests itself in a mosaic of liquid-ordered (Lo) or raft, and liquid-disordered (Ld) or non-raft...
PublicationsConformational changes of the H+-ATPase from Escherichia coli upon nucleotide binding detected by single molecule fluorescence
Using a confocal fluorescence microscope with an avalanche photodiode as detector, we studied the fluorescence of the tetramethylrhodamine labeled F-1 part of the H+-ATPase from Escherichia coli, EF1,...
PublicationsSTED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue
We have developed a bright, photostable, and far-red emitting fluorescent phosphoglycerolipid analogue to probe diffusion characteristics of lipids in membranes. The lipid analogue consists of a saturated...
PublicationsNew GM1 Ganglioside Derivatives for Selective Single and Double Labelling of the Natural Glycosphingolipid Skeleton
Selective single and double labelling of the natural ganglioside GM1 enables one to introduce various markers into different parts of the glycosphingolipid molecule without changing the natural skeleton....
PublicationsA Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy
Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared,...
PublicationsrsEGFP2 enables fast RESOLFT nanoscopy of living cells
The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While...
PublicationsA lipid bound actin meshwork organizes liquid phase separation in model membranes
The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation....
PublicationsHighly sensitive fluorescence detection technology currently available for HTS
Homogenous fluorescence methods are providing an important tool for HTS technologies. A wide range of different techniques have been established on the market, with read-outs ranging from total fluorescence...
PublicationsComparison of different fluorescence fluctuation methods for their use in FRET assays: Monitoring a protease reaction
We compare the accuracy of a variety of Fluorescence Fluctuation Spectroscopy (FFS) methods for the study of Forster Resonance Energy Transfer (FRET) assays. As an example, the cleavage of a doubly labeled,...
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