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Two-color far-field fluorescence nanoscopy based on photoswitchable emitters

We demonstrate two-color far-field fluorescence microscopy with nanoscale spatial resolution based on the photoswitching of individual fluorescent markers. By enabling, recording, and disabling the emission...

Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching

We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection...

Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis

The photostability of fluorescent dyes is of crucial importance for the statistical accuracy of single-molecule detection (SMD) and for the image quality of scanning confocal microscopy. Concurrent results...

Electroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes

Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods...

Analytical mode normalization and resonant state expansion for optical fibers – an efficient tool to model transverse disorder

We adapt the resonant state expansion to optical fibers such as capillary and photonic crystal fibers. As a key requirement of the resonant state expansion and any related perturbative approach, we derive...

Nanoscale Spatiotemporal Diffusion Modes Measured by Simultaneous Confocal and Stimulated Emission Depletion Nanoscopy Imaging

The diffusion dynamics in the cellular plasma membrane provides crucial insights into the molecular interactions, organization and bioactivity. Fluorescence correlation spectroscopy combined with super-resolution...

Editorial to fluorescence-correlation-spectroscopy (FCS)

An investigation on THz yield from laser-produced solid density plasmas at relativistic laser intensities

We experimentally characterize the generation of high-power terahertz radiation (THz) at the rear surface of a target irradiated by multiple laser pulses. A detailed dependence of the THz yield as a function...

Thin film superconducting quantum interferometer with ultralow inductance

A simple method has been developed for manufacturing a thin film superconducting quantum interferometer (SQI) with ultralow inductance (_10_13 H). Current-voltage and voltage-field characteristics of the...

Miniature diamond-anvil cells for FTIR-microspectroscopy of small quantities of biosamples

Fourier transform infrared (FTIR) spectroscopy techniques and data analyses have become widely available, are easy to use, and are convenient for studies of various bio samples, especially in biomedical...