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Fluorescence fluctuation spectroscopy in reduced detection volumes
Fluorescence fluctuation spectroscopy is a versatile technique applied to in vitro and in vivo investigations of biochemical processes Such as interactions, mobilities or densities with high specifity...
PublicationsMolecular photobleaching kinetics of rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy
Under high-excitation irradiance conditions in one- and two-photon induced fluorescence microscopy, the photostability of fluorescent dyes is of crucial importance for the detection sensitivity of single...
PublicationsFluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
During the lost decade for-field fluorescence microscopy methods have evolved that have resolution for below the wavelength of light. To outperform the limiting role of diffraction, all these methods,...
PublicationsEnhancing fluorescence brightness: Effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy
Experiments based on fluorescence detection ore limited by the population of the fluorescence marker's long-lived dark triplet state, leading to pronounced photobleaching reactions and blinking which reduces...
PublicationsHigh-Resolution Tracking of Single-Molecule Diffusion in Membranes by Confocalized and Spatially Differentiated Fluorescence Photon Stream Recording
The performance of a method is assessed which allows for the spatiotemporal tracking of single dye-labeled molecules during two-dimensional (2D) diffusional transits through the focal area of a modified...
PublicationsSpectral Imaging to Measure Heterogeneity in Membrane Lipid Packing
Physicochemical properties of the plasma membrane have been shown to play an important role in cellular functionality. Among those properties, the molecular order of the lipids, or the lipid packing, is...
PublicationsNew Fluorinated Rhodamines for Optical Microscopy and Nanoscopy
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated...
PublicationsRed-Emitting Rhodamine Dyes for Fluorescence Microscopy and Nanoscopy
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine...
PublicationsAstrocytes Resist HIV-1 Fusion but Engulf Infected Macrophage Material
HIV-1 disseminates to diverse tissues and establishes long-lived viral reservoirs. These reservoirs include the CNS, in which macrophage-lineage cells, and as suggested by many studies, astrocytes, may...
PublicationsSpectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order
Background: Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid...
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