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Homogeneity, transport, and signal properties of single Ag particles studied by single-molecule surface-enhanced resonance Raman scattering
We extended the sensitivity of Raman correlation spectroscopy in solution to the single-molecule level by applying surface- and resonance-enhanced Raman scattering (SERRS) combined with time-gated, confocal...
PublicationsAnalysis of photobleaching in single-molecule multicolor excitation and forster resonance energy transfer measurement
we investigated the influence of photobleaching in fluorescence experiments applying multicolor laser as well as Forster resonance energy transfer (FRET) mediated excitation using several red-emitting...
PublicationsStrategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
Given the particular importance of dye photostability for single-molecule and fluorescence fluctuation spectroscopy investigations, refined strategies were explored for how to chemically retard dye photobleaching....
PublicationsSTED microscopy of living cells – new frontiers in membrane and neurobiology
Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly...
PublicationsA comparative study on fluorescent cholesterol analogs as versatile cellular reporters
Cholesterol (Chol) is a crucial component of cellular membranes, but knowledge of its intracellular dynamics is scarce. Thus, it is of utmost interest to develop tools for visualization of Chol organization...
PublicationsMacrophages: micromanagers of antagonistic signaling nanoclusters
How cells integrate antagonistic receptor signaling events is enigmatic. Using superresolution optical microscopy, Lopes et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608094) demonstrate the...
PublicationsData registration and selective single-molecule analysis using multi-parameter fluorescence detection
A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence...
PublicationsDual-color total internal reflection fluorescence cross-correlation spectroscopy
We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy...
PublicationsMolecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron
Reversibly switchable fluorescent proteins can be repeatedly photoswitched between a fluorescent and a nonfluorescent state by irradiation with the light of two different wavelengths. The molecular basis...
PublicationsThe Efficacy of Raf Kinase Recruitment to the GTPase H-ras Depends on H-ras Membrane Conformer-specific Nanoclustering*.
Background: Ras nanoclusters contain 6-8 Ras proteins on the plasma membrane and serve as indispensable signaling platforms for Ras-MAPK signaling. Results: Ras membrane conformer mutants impart specific...
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