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A straightforward approach for gated STED-FCS to investigate lipid membrane dynamics
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches...
PublicationsSuper-resolution STED microscopy advances with yellow CW OPSL
Researchers have a growing need to push optical microscopy beyond the diffraction limit to answer key questions in biology, and stimulated emission depletion (STED) has proven to be a fluorescence imaging...
PublicationsOrientational and dynamical heterogeneity of rhodamine 6G terminally attached to a DNA helix revealed by NMR and single-molecule fluorescence spectroscopy
The comparison of Forster resonance energy transfer (FRET) efficiencies between two fluorophores covalently attached to a single protein or DNA molecule is an elegant approach for deducing information...
PublicationsConvergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS
Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of...
PublicationsDissecting the actin cortex density and membrane-cortex distance in living cells by super-resolution microscopy
Nanoscale spacing between the plasma membrane and the underlying cortical actin cytoskeleton profoundly modulates cellular morphology, mechanics, and function. Measuring this distance has been a key challenge...
PublicationsLaurdan and Di-4-ANEPPDHQ probe different properties of the membrane
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane lipid packing can be achieved using polarity sensitive probes whose emission spectrum depends on the lipid packing....
PublicationsGlycosylation and Lipids Working in Concert Direct CD2 Ectodomain Orientation and Presentation
Proteins embedded in the plasma membrane mediate interactions with the cell environment and play decisive roles in many signaling events. For cell-cell recognition molecules, it is highly likely that their...
PublicationsSingle-molecule identification of Coumarin-120 by time-resolved fluorescence detection: Comparison of one- and two-photon excitation in solution
Using two-photon excitation (TPE) at 700 nm as well as one-photon excitation (OPE) at 350 nm, we applied confocal fluorescence microscopy to detect single Coumarin-120 molecules in the solvents water and...
PublicationsQuantitative identification of different single molecules by selective time-resolved confocal fluorescence spectroscopy
Using a confocal epi-illuminated microscope together with a pulsed laser, new applications of the recently developed, real-time spectroscopic technique BIFL (burst integrated fluorescence lifetime) an...
PublicationsIdentification of single molecules in aqueous solution by time-resolved fluorescence anisotropy
Using a confocal epi-illuminated microscope with a polarizing beam splitter and dual-channel detection of single-molecule fluorescence induced by pulsed laser excitation, a new application of the three-dimensional,...
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