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A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization
Intramolecular photostabilization via triple-state quenching was recently revived as a tool to impart synthetic organic fluorophores with 'self-healing' properties. To date, utilization of such fluorophore...
PublicationsSelf-organizing actin patterns shape membrane architecture but not cell mechanics
Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic...
PublicationsA dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome
Many bacteria use a type III secretion system (T3SS) to inject effector proteins into host cells. Selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface...
PublicationsEnvelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state
Human immunodeficiency virus type 1 (HIV-1) assembles as immature particles, which require the proteolytic cleavage of structural polyprotein Gag and the clustering of envelope glycoprotein Env for infectivity....
PublicationsA near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine...
PublicationsPhotoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy
Fluorescent proteins that can be reversibly photoswitched between a fluorescent and a nonfluorescent state are important for innovative microscopy schemes, such as protein tracking(1), fluorescence resonance...
PublicationsA reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching...
PublicationsDirect observation of the nanoscale dynamics of membrane lipids in a living cell
Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events(1-5). Although several experiments indicate their existence,...
PublicationsDiffraction-unlimited all-optical imaging and writing with a photochromic GFP
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence...
PublicationsMulticolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to...
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