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Photoswitchable Spiropyran Dyads for Biological Imaging
The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photo chromic spironaphthothiopyran unit, which serves as a photoswitchable...
PublicationsNanoscale separation of molecular species based on their rotational mobility
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution,...
PublicationsAnalytical description of STED microscopy performance
Stimulated emission depletion (STED) resolves fluorescent features that are closer than the far-field optical diffraction limit by applying a spatially modulated light field keeping all but one of these...
PublicationsFluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)
We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion...
PublicationsSTED with wavelengths closer to the emission maximum
In stimulated emission depletion (STED) nanoscopy the wavelength of the STED beam is usually tuned towards the red tail of the emission maximum of the fluorophore. Shifting the STED wavelength closer to...
PublicationsSingle-molecule detection of coumarin-120
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched...
PublicationsExploring single-molecule dynamics with fluorescence nanoscopy
The study of molecular dynamics at the single-molecule level with fluorescence correlation spectroscopy (FCS) and far-field optics has contributed greatly to the functional understanding of complex systems....
PublicationsDark state photophysics of nitrogen-vacancy centres in diamond
Nitrogen-vacancy (NV) colour centres in diamond are attractive fluorescence emitters owing to their unprecedented photostability and superior applicability to spin manipulation and sub-diffraction far-field...
PublicationsNanoscopy of Living Brain Slices with Low Light Levels
Lens-based fluorescence microscopy, which has long been limited in resolution to about 200 nanometers by diffraction, is rapidly evolving into a nanoscale imaging technique. Here, we show that the superresolution...
PublicationsPhosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment
Synaptic-vesicle exocytosis is mediated by the vesicular Ca2+ sensor synaptotagmin-1. Synaptotagmin-1 interacts with the SNARE protein syntaxin-1A and acidic phospholipids such as phosphatidylinositol...
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