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The mystery of membrane organization: composition, regulation and roles of lipid rafts
Cellular plasma membranes are laterally heterogeneous, featuring a variety of distinct subcompartments that differ in their biophysical properties and composition. A large number of studies have focused...
PublicationsDissection of mechanical force in living cells by super-resolved traction force microscopy
Cells continuously exert or respond to mechanical force. Measurement of these nanoscale forces is a major challenge in cell biology; yet such measurement is essential to the understanding of cell regulation...
PublicationsSTED microscopy reveals crystal colour centres with nanometric resolution
Because they have spin states that can be optically polarized and detected, fluorescent nitrogen vacancies in diamond(1-3) have considerable potential for applications in quantum cryptography(4,5) and...
PublicationsMajor signal increase in fluorescence microscopy through dark-state relaxation
We report a substantial signal gain in fluorescence microscopy by ensuring that transient molecular dark states with lifetimes > 1 mu s, such as the triplet state relax between two molecular absorption...
PublicationsFluorescence nanoscopy by ground-state depletion and single-molecule return
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left...
PublicationsSharper low-power STED nanoscopy by time gating
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details...
PublicationsNanoscopy with more than 100,000 ‘doughnuts’
We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently...
PublicationsMulti-protein assemblies underlie the mesoscale organization of the plasma membrane
Most proteins have uneven distributions in the plasma membrane. Broadly speaking, this may be caused by mechanisms specific to each protein, or may be a consequence of a general pattern that affects the...
PublicationsScanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells
The interaction of lipids and proteins plays an important role in plasma membrane bioactivity, and much can be learned from their diffusion characteristics. Here we present the combination of super-resolution...
PublicationsHydrophobic mismatch sorts SNARE proteins into distinct membrane domains
The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic...
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