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Combination of Patch Clamp and Raman Spectroscopy for Single-Cell Analysis
In this contribution we present the combination of patch clamp with Raman spectroscopy for a label-free quantitative detection of intracellular components. Patch clamp is used to gain controlled access...
PublicationsExcited-State Dynamics of Protochlorophyllide Revealed by Subpicosecond Infrared Spectroscopy
To gain a better understanding of the light-induced reduction of protochlorophyllide (PChlide) to chlorophyllide as a key regulatory step in chlorophyll synthesis, we performed transient infrared absorption...
PublicationsTowards a fast, high specific and reliable discrimination of bacteria on strain level by means of SERS in a microfluidic device
The interest in a fast, high specific and reliable detection method for bacteria dentification is increasing. We will show that the application of vibrational spectroscopy is feasible for the validation...
PublicationsThe molecular mechanism of dual emission in terpyridine transition metal complexes—ultrafast investigations of photoinduced dynamics
Temperature dependent luminescence experiments are combined with femtosecond time-resolved transient absorption spectroscopy to decipher the photoinduced excited-state relaxation pathway in mononuclear...
PublicationsPhotoluminescence and Raman Scattering in Wet Chemically Etched Silicon Nanowires
Silicon nanowires with mean diameters of about 50-100 nm formed by wet-chemical etching of crystalline silicon wafers with low and high doping level were investigated by means of photoluminescence and...
PublicationsMagnetic and fluorescent core–shell nanoparticles for ratiometric pH sensing
This paper describes the preparation of nanoparticles (NPs) with a magnetic core surrounded by two successive silica shells embedding two fluorophores which allow ratiometric pH-measurments in the biologically...
PublicationsSensoric potential of gold-silver core-shell nanoparticles
The sensitivities of five different core-shell nanostructures were investigated towards changes in the refractive index of the surrounding medium. The shift of the LSPR maximum served as a measure of the...
PublicationsInvestigation of adhesive-dentin interfaces using Raman microspectroscopy and small angle X-ray scattering
Human dentin specimens were treated with two different etch-and-rinse adhesives, Single Bond 2 (SB2) and Prime bond NT (PBNT), and two composite resins, TPH and P60. Cross-sectional samples, approximately...
PublicationsComparison of numerical methods in near-field computation for metallic nanoparticles
Four widely used electromagnetic field solvers are applied to the problem of scattering by a spherical or spheroidal silver nanoparticle in glass. The solvers are tested in a frequency range where the...
PublicationsSpectral Unmixing and Clustering Algorithms for assessment of single cells by Raman Microscopic Imaging
A detailed comparison of six multivariate algorithms is presented to analyze and generate Raman microscopic images that consist of a large number of individual spectra. This includes the segmentation algorithms...
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