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DNA-Microarray-based Genotyping of Clostridium difficile

Background: Clostridium difficile can cause antibiotic-associated diarrhea and a possibility of outbreaks in hospital settings warrants molecular typing. A microarray was designed that included toxin genes...

Characterisation of MRSA strains isolated from patients in a hospital in Riyadh, Kingdom of Saudi Arabia

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is spreading worldwide and poses a serious public health problem, being present in hospital settings and communities. However, from the Middle...

Evolution and diversity of community-associated methicillin-resistant Staphylococcus aureus in a geographical region

Background: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) was first reported in remote regions of Western Australia and is now the predominant MRSA isolated in the state. The...

Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes

Background: The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian...

An alternative method to amplify RNA without loss of signal conservation for expression analysis with a proteinase DNA microarray in the ArrayTube (R) format

Background: Recent developments in DNA microarray technology led to a variety of open and closed devices and systems including high and low density microarrays for high-throughput screening applications...

Single-step purification of T7 RNA polymerase with a 6-histidine tag

Complex patterns predicted in an in vitro experimental model system for the evolution of molecular cooperation

An isothermal biochemical in vitro amplification system with two trans-cooperatively coupled amplifying DNA molecules was investigated homogeneously using a hierarchy of kinetic models and as a simplified...

Residual DNA in thermostable DNA polymerases – a cause of irritation in diagnostic PCR and microarray assays

In a validation trial of a DNA microarray test for chlamydiae we repeatedly observed false-positive PCR amplicons from truly negative samples and non-template controls. Various PCR tests, microarray hybridization...

Purification and properties of L(-)-carnitine dehydrogenase from Agrobacterium sp.

l(−)-Carnitine:NAD+ oxidoreductase, EC 1.1.1.108, from Agrobacterium sp. catalyzes the oxidation of l(−)-carnitine to 3-dehydrocarnitine as initial step of l(−)-carnitine degradation. The enzyme...

Phänotypisierung und Microarraybasierte Genotypisierung des Antibiotika-Resistenzverhaltens von MRSA und MSSA aus Viertelgemelksproben klinisch gesunder Milchkühe [Phenotyping and microarray based genotyping of the antibiotic resistance of MRSA and MSSA from quarter milk samples of clinically healthy dairy cows]

Ziel dieser Untersuchung war es, in einer systematischen Stichprobe von Staphy- lococcus aureus-Isolaten aus Rohmilch klinisch gesunder Kühe eine umfassende Charakterisierung des Antibiotika-Resistenzverhaltens...