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A multiplex real-time PCR for the direct, fast, economic and simultaneous detection of the carbapenemase genes blaKPC, blaNDM, blaVIM and blaOXA-48

A novel multiplex real-time PCR was designed to detect the clinically most important carbapenemase genes, blaKPC, blaVIM, blaNDM and blaOXA-48. The multiplex assay was verified testing genomic DNA of 24...

Serogenotyping and emergence of extended-spectrum beta-lactamase genes in non-typhoidal Salmonella: first report from Saudi Arabia

Genotyping of Staphylococcus aureus associated with nasal colonization among healthcare workers using DNA microarray

Introduction: Healthcare workers (HCWs) colonized with Staphylococcus aureus may serve as a reservoir of infection. This study was carried to determine the genetic make-up of S. aureus nasal colonizers...

In vitro activity of ceftaroline against mecC-positive MRSA isolates

Ceftaroline is a new cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA). A collection of 17 clinical and veterinary mecC-positive MRSA isolates was tested to evaluate...

Staphylococci in cattle and buffaloes with mastitis in Dakahlia Governorate, Egypt

The aim of this study was to provide the first detailed insight into the population structure of Staphylococcus aureus in one modern dairy farm (Gamasa) and several household cows and buffaloes in Dakahlia...

Multiple cases of methicillin-resistant CC130 Staphylococcus aureus harboring mecC in milk and swab samples from a Bavarian dairy herd

The discovery of a new mecA homolog, mecC, necessitates a modification of diagnostic procedures for the identification of methicillin-resistant Staphylococcus aureus (MRSA), as most assays used for the...

Molecular characteristics of bap-positive Staphylococcus aureus strains from dairy cow mastitis

The biofilm-associated protein (Bap) of Staphylococcus aureus is a high molecular weight cell-wallanchored protein involved in biofilm formation, first described in bovine mastitis strains from Spain....

DNA Microarray-Based Typing of Streptococcus agalactiae Isolates

Streptococcus agalactiae frequently colonizes the urogenital tract, and it is a major cause of bacterial septicemia, meningitis, and pneumonia in newborns. For typing purposes, a microarray targeting group...

DNA Microarray-Based PCR Ribotyping of Clostridium difficile

This study presents a DNA microarray-based assay for fast and simple PCR ribotyping of Clostridium difficile strains. Hybridization probes were designed to query the modularly structured intergenic spacer...

Development of a Rapid Microarray-Based DNA Subtyping Assay for the Alleles of Shiga Toxins 1 and 2 of Escherichia coli

In this study, we developed a new rapid, economic, and automated microarray-based genotyping test for the standardized subtyping of Shiga toxins 1 and 2 of Escherichia coli. The microarrays from Alere...