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- Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies
Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies
in: Molecular and Cellular Probes (2006)
This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples.