Collaborative project: "Development of a modular multiplex detection system for plasmid-based antibiotic resistance monitoring (PbR - plasmid-based resistance detection)" Subproject: "Development of a multiplex detection system of resistance genes by separation of natural plasmids from complex samples and subsequent detection of plasmid antibiotic resistance genes on a plasmonic microarray chip (PlasmidChip)"
Microbial antibiotic resistance is an increasing worldwide problem for patient care. The resistance is spread via independently replicating mobile plasmids, which are even exchanged between the bacteria across species. The main focus of the available monitoring systems is on hospitals. Natural plasmids, which are mainly exchanged between the pathogens in the environment, are currently not depicted.
The joint project therefore aims to develop a plasmid-based antibiotic resistance monitoring system. The aim is to use an innovative plasmid isolation method that is integrated into a microfluidic chip. Resistance testing will then be carried out, also integrated on the microfluidic chip, using a highly innovative and label-free plasmonic method. The signals will be read using imaging spectrometry. The joint project will benefit from the close integration of the experts and the coordination by the industrial partner integrating the entire system.
The project is supported by the Free State of Thuringia under the number 2018 FE 9039 and co-financed by funds of the European Union within the framework of the European Regional Development Fund (ERDF).
SIOS Meßtechnik GmbH, Ilmenau (Projektkoordinator)
Mildendo GmbH, Jena
Universitätsklinikum Jena, Institut für Infektionsmedizin und Krankenhaushygiene