Loop mediated amplification as promising on-site detection approach for Legionella pneumophila and Legionella spp.

in: Applied Microbiology and Biotechnology (2020)
Reuter, Cornelia; Slesiona, Nicole; Hentschel, Stefanie; Aehlig, Oliver; Breitenstein, Antje; Csáki, Andrea; Fritzsche, Wolfgang
Recently Legionella pneumophila is the main causative waterborne organism of severe respiratory infections. Additionally, other Legionella species are documented as human pathogens. In our work we describe a rapid detection method which combines two advantages for sensitive and specific detection of the genus Legionella: the fast isothermal amplification method “Loop-mediated isothermal AMPlification” (LAMP), and a colorimetric detection method using the metal indicator hydroxynaphtol blue (HBN) which allows to determine an optical signal with a simple read out (with the naked eye). Moreover, we present two approaches for minimizing the assay volume using a stationary microchip LAMP and droplet digital based LAMP (ddLAMP) as promising high sensitive setups.

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