Silicon-rhodamine isothiocyanate for fluorescent labelling†

in: Organic & Biomolecular Chemistry (2021)
Nasufovic, Veselin; Then, Patrick; Dröge, Fabian; Duonq, Michael; Kaether, Christoph; Dietzek, Benjamin; Heintzmann, Rainer; Arndt, Hans-Dieter
An efficient synthesis for silicon-rhodamines was developed, enabling the preparation and evaluation of silicon-rhodamine isothiocyanate (SITC) as a novel tool for facile fluorescent labeling. Ease of use in conjugation to amino groups, high stability and excellent photophysical properties are demonstrated. SITC-actin was found to be neutral to F-actin polymerization induction and well suited for high resolution fluorescence microscopy.

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