Selective plane activation structured illumination microscopy

in: BioRxiv (2023)
Temma, Kenta; Oketani, Ryosuke; Kubo, Toshiki; Bando, Kazuki; Maeda, Kazuhiko; Sugiura, Kazunori; Matsuda, Tomoki; Heintzmann, Rainer; Kaminishi, Tatsuya; Fukuda, Koki; Hamasaki, Maho; Nagai, Takeharu; Fujita, Katsumasa
Resolution enhancement in structured illumination microscopy is hindered when volumetric samples are observed owing to background signals from out-of-focus planes. In this study, we utilized selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. We demonstrated highresolution fluorescence imaging of volumetric samples, including dense fluorescent objects in a cell spheroid.

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