Lithographically patterned silicon nanowire arrays for matrix free LDI-TOF/MS analysis of lipids

in: Lab on a Chip (2010)
Muck, Alexander; Hübner, Uwe; Christiansen, Silke; Svatoš, Aleš; Stelzner, Thomas
Silicon nanowire arrays were patterned onto silicon chips by a combination of lithography and chemical vapor deposition using the vapor-liquid-solid growth method. Thus, highly reproducible sample deposition zones were obtained that were used for laser desorption ionization (LDI) mass spectrometric analysis of lipidic species with lithium salts as dopants. Using a conventional UV laser (337 nm), hydrocarbons and numerous lipids (triglycerides, diglycerides, wax esters) could be effectively lithiated yielding [M + Li]+ ions. Upon doping with lithium hydroxide the SiNW arrays yielded high signal-to-noise ratios with low limits of detection (e.g. 750 pg tripalmitin on target with S/N 5) and efficient ionization for a range of fatty acids (FA), mono-, di- and triglycerides and hydrocarbons (HC), in the form of [FA _ H + 2Li]+, [mono- or diglyceride _ H2O + Li]+, or [triglyceride + Li]+ and [HC + Li]+, respectively. It is expected that these chips will find a broad range of applications in the analysis of natural compounds and food control.

Third party cookies & scripts

This site uses cookies. For optimal performance, smooth social media and promotional use, it is recommended that you agree to third party cookies and scripts. This may involve sharing information about your use of the third-party social media, advertising and analytics website.
For more information, see privacy policy and imprint.
Which cookies & scripts and the associated processing of your personal data do you agree with?

You can change your preferences anytime by visiting privacy policy.