Clinical Spectroscopic Diagnostics

The reaction of (OC)5MnBr with bis(3,5-dimethyl-1-pyrazolyl)methane yields [{(PzMe2)2CH2}Mn(CO)3Br](1). The use of tridentate heteroscorpionates such as bis(3,5-dimethyl-1-pyrazolyl)acetic acid and 2,2-bis(3,5-dimethyl-1-pyrazolyl)ethanol leads to the formation of mononuclear [(OC)3Mn{(PzMe2)2CH–CO2}](2) and [(OC)3Mn{(PzMe2)2CH–CH2OH}]Br (3, CORM-ONN1). Salt-like photoCORM 3 is soluble in aqueous media up to a concentration of 200 μM, non-toxic up to an approx. 65 μM solution and releases all carbonyl ligands upon irradiation. The molecular complexes 1 and 2 are insoluble in aqueous solutions. CORM-ONN1 (3) slowly degrades in methanol yielding iCORM 4, consisting of the complex cation [{(PzMe2)2CH–CH2OH}{(PzMe2)2CH–CH2O}Mn]+ and the [Mn(CO)5]− counter anion with the cations linked to a dimeric unit by intermolecular hydrogen bridges between the alcohol and alkoxide functionalities.