The scientific profile of the research group Biopolarisation aims at the application and further development of a novel technological approach, which utilizes the interaction of the electromagnetic near-field of optically polarised biological species with a conductive tip of an atomic force microscope for characterizing the biological species. The signal evaluation using Force Microscopy is independent of noisy scattering light which otherwise contributes to the signal in the frequently used optical evaluation methods. Thus, it provides accuracies enhanced by several orders of magnitude. The new scientific approach complements investigation methods for the characterization of biological species that are already established at the Leibniz-IPHT. In particular it will be exploited in collaboration with the Clinical Spectroscopic Diagnostics group.
Additionally, we apply and improve optical polarization resolved fluorescence microscopy imaging techniques, in particular, two-dimensional polarisation imaging (2D POLIM), developed in the group of Ivan Scheblykin in Lund, for structure determination ex vivo in biological tissue and biotechnical materials in the range from mm to nm.
A further scientific topic is the characterization and improvement of chips with implanted pattern of near surface charges. Preliminary investigation of such PolCarrTM-Chips demonstrated stable adsorption of DEAE-DEXTRAN(+) at the implanted region. This opens up a large variety of promising possibilities for specific adsorption of biological species. In particular, it allows for characterization of biological matter directly adsorbed from solution without the necessity of drying the sample.
Gained interest? Our group is developing and we are looking forward to further collaborations. In particular, we welcome students for Bachelor and Master projects.