Complexity of living matter currently poses the most significant barrier to modern in-vivo microscopy. Fueled by various branches of life sciences, the race is now to increase the penetration depth of super-resolution imaging inside living organisms. Additionally, no high-resolution in-vivo imaging technique has ever been introduced into medical, particularly surgical practice.Amongst other activities we develop a new class of endoscopes that can break through this barrier. This technology can potentially go as far as reaching super-resolution with instruments having a footprint comparable to the dimensions of a single cell. Project LIFEGATE (“Holographic super-resolution micro-endoscopy for in-vivo applications”) is funded as Consolidator Grant by the European Research Council (ERC).
Our transdisciplinary team is now pushing the fundamental and technological limits of the enabling principle - holographic control of light propagation in multimode fibres. Through advanced analytical and numerical modelling, and major advancements of experimental methods, our team is developing a powerful platform for fast and efficient recovery of randomized imagery, retrieved from both rigid and flexible single-fibre endoscopes. This ‘gate-through-life’ could enable our team to deploy several prominent light-based imaging methods, including super-resolution approaches, inside freely moving animal models and ultimately humans.