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- Fluorescence nanoscopy by ground-state depletion and single-molecule return
Fluorescence nanoscopy by ground-state depletion and single-molecule return
in: Nature Methods (2008)
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
DOI: 10.1038/nmeth.1257