Raman spectroscopic detection of physiology changes in plasmid-bearing Escherichia coli with and without antibiotic treatment

in: Analytical and Bioanalytical Chemistry (2011)
Walter, Angela; Bocklitz, Thomas W.; Schumacher, Wilm; Rösch, Petra; Kothe, Erika; Popp, Jürgen; Reinicke, Martin
Bacterial resistances against antibiotics are increasingly problematic for medical treatment of pathogenic bacteria, e.g., in hospitals. Resistances are, among other genes, often encoded on plasmids which can be transmitted between bacteria not only within one species, but also between different species, genera, and families. The plasmid pDrive is transformed into bacteria of the model strain Escherichia coli DH5α. Within this investigation, we applied micro-Raman spectroscopy with two different excitation wavelengths in combination with support vector machine (SVM) and linear discriminant analysis (LDA) to differentiate between bacterial cultures according to their cultural plasmid content. Recognition rates of about 92% and 90% are achieved by Raman excitation at 532 and 244 nm, respectively. The SVM loadings reveal that the pDrive transformed bacterial cultures exhibit a higher DNA content.

DOI: Array

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