Clinical Spectroscopic Diagnostics

The water insoluble and photoactive CO releasing molecule dimanganese decacarbonyl (CORM-1) has been non-covalently embedded into poly(L-lactide-co-D/L-lactide) nanofibers via electrospinning to enable bioavailability and water accessibility of CORM-1. SEM images of the resulting hybrid non-wovens reveal a po-rous nanofiber morphology. IR spectra show the same set of carbonyl bands for the CORM-1 precursor and the non-woven. When the material was exposed to light (365-480 nm) CO release from the incorporated CORM-1 was observed via heterogeneous myoglobin assay, portable CO electrode and IR gas cuvette. The CO release rate was wavelength dependent. Irradiation at 365 nm resulted in a four times faster release than at 480 nm. 3.3 µmol of CO per mg non-woven can be generated. Mouse fibroblast 3T3 cells were used to show the hybrid material to be non-toxic in the darkness and strongly photocytotoxic when light is applied