A new human adipocyte model with PTEN haploinsufficiency

in: Adipocyte (2020)
Kässner, Franziska; Kirstein, Anna; Händel, Norman; Schmid, Gordian L.; Landgraf, Kathrin; Berthold, Antje; Tannert, Astrid; Schaefer, Michael; Wabitsch, Martin; Kiess, Wieland; Körner, Antje; Garten, Antje
Few human cell strains are suitable and readily available as in vitro adipocyte models. We used resected lipoma tissue from a patient with germline phosphatase and tensin homolog (PTEN) haploinsufficiency to establish a preadipocyte cell strain termed LipPD1 and aimed to characterize cellular functions and signalling pathway alterations in comparison to the established adipocyte model Simpson-Golabi-Behmel-Syndrome (SGBS) and to primary stromal-vascular fraction cells. We found that both cellular life span and the capacity for adipocyte differentiation as well as adipocytespecific functions were preserved in LipPD1 and comparable to SGBS adipocytes. Basal and growth factor-stimulated activation of the PI3 K/AKT signalling pathway was increased in LipPD1 preadipocytes, corresponding to reduced PTEN levels in comparison to SGBS cells. Altogether, LipPD1 cells are a novel primary cell model with a defined genetic lesion suitable for the study of adipocyte biology.

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