Structured illumination microscopy of autofluorescent aggregations in human tissue

in: Micron (2011)
Heintzmann, Rainer; Best, Gerrit; Amberger, Roman; Baddeley, David; Ach, Thomas; Dithmar, Stefan; Cremer, Christoph
Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SIM) using a specially designed microscope setup. In this microscope the structured illumination was generated with a Twyman-Green Interferometer. This SIM technique allowed us to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. In this work the unique setup of the microscope made possible the application of SIM with three different excitation wavelengths (488, 568 and 647nm), thus enabling us to gather spectral information about the autofluorescence signal.

DOI: Array

Third party cookies & scripts

This site uses cookies. For optimal performance, smooth social media and promotional use, it is recommended that you agree to third party cookies and scripts. This may involve sharing information about your use of the third-party social media, advertising and analytics website.
For more information, see privacy policy and imprint.
Which cookies & scripts and the associated processing of your personal data do you agree with?

You can change your preferences anytime by visiting privacy policy.