Chiral Structured Illumination Microscopy

in: ACS Photonics (2021)
Huang, Jer-Shing; Huang, Shiang-Yu; Heintzmann, Rainer; Förster, Ronny; Karras, Christian; Zhang, Jiwei
We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism, and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the circular dichroism dependent fluorescence is detected. With image reconstruction, the spatial distribution of the chiral domains can be obtained at subdiffraction-limited resolution. We theoretically demonstrate this method and discuss the feasibility of using an optical chirality engineering approach based on far-field optics.

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