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Spectral Imaging to Measure Heterogeneity in Membrane Lipid Packing
Physicochemical properties of the plasma membrane have been shown to play an important role in cellular functionality. Among those properties, the molecular order of the lipids, or the lipid packing, is...
PublikationenNew Fluorinated Rhodamines for Optical Microscopy and Nanoscopy
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated...
PublikationenRed-Emitting Rhodamine Dyes for Fluorescence Microscopy and Nanoscopy
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine...
PublikationenAstrocytes Resist HIV-1 Fusion but Engulf Infected Macrophage Material
HIV-1 disseminates to diverse tissues and establishes long-lived viral reservoirs. These reservoirs include the CNS, in which macrophage-lineage cells, and as suggested by many studies, astrocytes, may...
PublikationenSpectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order
Background: Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, and lateral lipid...
PublikationenFluorescence intensity and lifetime distribution analysis: Toward higher accuracy in fluorescence fluctuation spectroscopy
Fluorescence fluctuation methods such as fluorescence correlation spectroscopy and fluorescence intensity distribution analysis (FIDA) have proven to be versatile tools for studying molecular interactions...
PublikationenRapid analysis of Forster resonance energy transfer by two-color global fluorescence correlation spectroscopy: Trypsin proteinase reaction
In this study we introduce the combination of two-color global fluorescence correlation spectroscopy (2CG-FCS) and Forster resonance energy transfer (FRET) as a very powerful combination for monitoring...
PublikationenTwo-color far-field fluorescence nanoscopy
We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and...
PublikationenFluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the...
PublikationenGeneration of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy
Reversibly switchable fluorescent proteins (RSFPs) are GFP-like proteins that may be repeatedly switched by irradiation with light from a fluorescent to a nonfluorescent state, and vice versa. They can...
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