6814 Suchergebnisse für: „“
Regulation of peroxisomal matrix protein import by ubiquitination
Peroxisomes are organelles that play an important role in many cellular tasks. The functionality of peroxisomes depends on the proper import of their matrix proteins. Peroxisomal matrix proteins are imported...
PublikationenPartitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes
Several simplified membrane models featuring coexisting liquid disordered (Ld) and ordered (Lo) lipid phases have been developed to mimic the heterogeneous organization of cellular membranes, and thus,...
PublikationenClosing the gap: The approach of optical and computational microscopy to uncover biomembrane organization
Biological membranes are complex composites of lipids, proteins and sugars, which catalyze a myriad of vital cellular reactions in a spatiotemporal tightly controlled manner. Our understanding of the organization...
Publikationen1.8 angstrom bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants
RSFPs (reversibly switchable fluorescent proteins) may be repeatedly converted between a fluorescent and a non-fluorescent state by irradiation and have attracted widespread interest for many new applications....
PublikationenDetection and characterization of single molecules in aqueous solution
Using a confocal microscope with a single-photon avalanche photodiode as detector, we studied photon bursts of single Rhodamine 6G (R6G) and Rhodamin B-zwitterion (RE) molecules in aqueous solution by...
PublikationenTwo-color far-field fluorescence nanoscopy based on photoswitchable emitters
We demonstrate two-color far-field fluorescence microscopy with nanoscale spatial resolution based on the photoswitching of individual fluorescent markers. By enabling, recording, and disabling the emission...
PublikationenResolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching
We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection...
PublikationenPhotobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis
The photostability of fluorescent dyes is of crucial importance for the statistical accuracy of single-molecule detection (SMD) and for the image quality of scanning confocal microscopy. Concurrent results...
PublikationenElectroformation of Giant Unilamellar Vesicles on Stainless Steel Electrodes
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electroswelling, is one of the most prevalent methods...
PublikationenAnalytical mode normalization and resonant state expansion for optical fibers – an efficient tool to model transverse disorder
We adapt the resonant state expansion to optical fibers such as capillary and photonic crystal fibers. As a key requirement of the resonant state expansion and any related perturbative approach, we derive...
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