6814 Suchergebnisse für: „“
Characterization of Horizontal Lipid Bilayers as a Model System to Study Lipid Phase Separation
Artificial lipid membranes are widely used as a model system to study single ion channel activity using electrophysiological techniques. In this study, we characterize the properties of the artificial...
PublikationenMulticolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength
Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction...
PublikationenSTED Nanoscopy Reveals Molecular Details of Cholesterol- and Cytoskeleton-Modulated Lipid Interactions in Living Cells
Details about molecular membrane dynamics in living cells, such as lipid-protein interactions, are often hidden from the observer because of the limited spatial resolution of conventional far-field optical...
PublikationenMembrane Orientation and Lateral Diffusion of BODIPY-Cholesterol as a Function of Probe Structure
Cholesterol tagged with the BODIPY fluorophore via the central difluoroboron moiety of the dye (B-Chol) is a promising probe for studying intracellular cholesterol dynamics. We synthesized a new BODIPY-cholesterol...
PublikationenReorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy
Myelin is a multilayered membrane that ensheathes axonal fibers in the vertebrate nervous system, allowing fast propagation of nerve action potentials. It contains densely packed lipids, lacks an actin-based...
PublikationenPolarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy
The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids...
PublikationenModulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles
The transfer of material from phospholipid-coated microbubbles to cell membranes has been hypothesized to play a role in ultrasound-mediated drug delivery. In this study, we employed quantitative fluorescence...
PublikationenCritical importance of appropriate fixation conditions for faithful imaging of receptor microclusters
Receptor clustering is known to trigger signalling events that contribute to critical changes in cellular functions. Faithful imaging of such clusters by means of fluorescence microscopy relies on the...
PublikationenFoCuS-point: software for STED fluorescence correlation and time-gated single photon counting
Motivation: Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility and how mobility relates to molecular interaction dynamics and bioactivity in living cells. The...
PublikationenWhy do peroxisomes associate with the cytoskeleton?
Attachment of peroxisomes to cytoskeleton and movement along microtubular filaments and actin cables are essential and highly regulated processes enabling metabolic efficiency, biogenesis, maintenance...
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