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Macrophages: micromanagers of antagonistic signaling nanoclusters
How cells integrate antagonistic receptor signaling events is enigmatic. Using superresolution optical microscopy, Lopes et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608094) demonstrate the...
PublikationenData registration and selective single-molecule analysis using multi-parameter fluorescence detection
A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence...
PublikationenDual-color total internal reflection fluorescence cross-correlation spectroscopy
We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy...
PublikationenMolecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron
Reversibly switchable fluorescent proteins can be repeatedly photoswitched between a fluorescent and a nonfluorescent state by irradiation with the light of two different wavelengths. The molecular basis...
PublikationenThe Efficacy of Raf Kinase Recruitment to the GTPase H-ras Depends on H-ras Membrane Conformer-specific Nanoclustering*.
Background: Ras nanoclusters contain 6-8 Ras proteins on the plasma membrane and serve as indispensable signaling platforms for Ras-MAPK signaling. Results: Ras membrane conformer mutants impart specific...
PublikationenSuper-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins
Membrane-associated events during peroxisomal protein import processes play an essential role in peroxisome functionality. Many details of these processes are not known due to missing spatial resolution...
PublikationenPhase Partitioning of GM1 and Its Bodipy-Labeled Analog Determine Their Different Binding to Cholera Toxin
Driven by interactions between lipids and proteins, biological membranes display lateral heterogeneity that manifests itself in a mosaic of liquid-ordered (Lo) or raft, and liquid-disordered (Ld) or non-raft...
PublikationenConformational changes of the H+-ATPase from Escherichia coli upon nucleotide binding detected by single molecule fluorescence
Using a confocal fluorescence microscope with an avalanche photodiode as detector, we studied the fluorescence of the tetramethylrhodamine labeled F-1 part of the H+-ATPase from Escherichia coli, EF1,...
PublikationenSTED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue
We have developed a bright, photostable, and far-red emitting fluorescent phosphoglycerolipid analogue to probe diffusion characteristics of lipids in membranes. The lipid analogue consists of a saturated...
PublikationenNew GM1 Ganglioside Derivatives for Selective Single and Double Labelling of the Natural Glycosphingolipid Skeleton
Selective single and double labelling of the natural ganglioside GM1 enables one to introduce various markers into different parts of the glycosphingolipid molecule without changing the natural skeleton....
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