6814 Suchergebnisse für: „“
A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy
Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared,...
PublikationenrsEGFP2 enables fast RESOLFT nanoscopy of living cells
The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While...
PublikationenA lipid bound actin meshwork organizes liquid phase separation in model membranes
The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation....
PublikationenHighly sensitive fluorescence detection technology currently available for HTS
Homogenous fluorescence methods are providing an important tool for HTS technologies. A wide range of different techniques have been established on the market, with read-outs ranging from total fluorescence...
PublikationenComparison of different fluorescence fluctuation methods for their use in FRET assays: Monitoring a protease reaction
We compare the accuracy of a variety of Fluorescence Fluctuation Spectroscopy (FFS) methods for the study of Forster Resonance Energy Transfer (FRET) assays. As an example, the cleavage of a doubly labeled,...
PublikationenFluorescence fluctuation spectroscopy in reduced detection volumes
Fluorescence fluctuation spectroscopy is a versatile technique applied to in vitro and in vivo investigations of biochemical processes Such as interactions, mobilities or densities with high specifity...
PublikationenMolecular photobleaching kinetics of rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy
Under high-excitation irradiance conditions in one- and two-photon induced fluorescence microscopy, the photostability of fluorescent dyes is of crucial importance for the detection sensitivity of single...
PublikationenFluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
During the lost decade for-field fluorescence microscopy methods have evolved that have resolution for below the wavelength of light. To outperform the limiting role of diffraction, all these methods,...
PublikationenEnhancing fluorescence brightness: Effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy
Experiments based on fluorescence detection ore limited by the population of the fluorescence marker's long-lived dark triplet state, leading to pronounced photobleaching reactions and blinking which reduces...
PublikationenHigh-Resolution Tracking of Single-Molecule Diffusion in Membranes by Confocalized and Spatially Differentiated Fluorescence Photon Stream Recording
The performance of a method is assessed which allows for the spatiotemporal tracking of single dye-labeled molecules during two-dimensional (2D) diffusional transits through the focal area of a modified...
Publikationen