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Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane lipid packing can be achieved using polarity sensitive probes whose emission spectrum depends on the lipid packing....
PublikationenGlycosylation and Lipids Working in Concert Direct CD2 Ectodomain Orientation and Presentation
Proteins embedded in the plasma membrane mediate interactions with the cell environment and play decisive roles in many signaling events. For cell-cell recognition molecules, it is highly likely that their...
PublikationenSingle-molecule identification of Coumarin-120 by time-resolved fluorescence detection: Comparison of one- and two-photon excitation in solution
Using two-photon excitation (TPE) at 700 nm as well as one-photon excitation (OPE) at 350 nm, we applied confocal fluorescence microscopy to detect single Coumarin-120 molecules in the solvents water and...
PublikationenQuantitative identification of different single molecules by selective time-resolved confocal fluorescence spectroscopy
Using a confocal epi-illuminated microscope together with a pulsed laser, new applications of the recently developed, real-time spectroscopic technique BIFL (burst integrated fluorescence lifetime) an...
PublikationenIdentification of single molecules in aqueous solution by time-resolved fluorescence anisotropy
Using a confocal epi-illuminated microscope with a polarizing beam splitter and dual-channel detection of single-molecule fluorescence induced by pulsed laser excitation, a new application of the three-dimensional,...
PublikationenHomogeneity, transport, and signal properties of single Ag particles studied by single-molecule surface-enhanced resonance Raman scattering
We extended the sensitivity of Raman correlation spectroscopy in solution to the single-molecule level by applying surface- and resonance-enhanced Raman scattering (SERRS) combined with time-gated, confocal...
PublikationenAnalysis of photobleaching in single-molecule multicolor excitation and forster resonance energy transfer measurement
we investigated the influence of photobleaching in fluorescence experiments applying multicolor laser as well as Forster resonance energy transfer (FRET) mediated excitation using several red-emitting...
PublikationenStrategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
Given the particular importance of dye photostability for single-molecule and fluorescence fluctuation spectroscopy investigations, refined strategies were explored for how to chemically retard dye photobleaching....
PublikationenSTED microscopy of living cells – new frontiers in membrane and neurobiology
Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly...
PublikationenA comparative study on fluorescent cholesterol analogs as versatile cellular reporters
Cholesterol (Chol) is a crucial component of cellular membranes, but knowledge of its intracellular dynamics is scarce. Thus, it is of utmost interest to develop tools for visualization of Chol organization...
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