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Super-Resolved Traction Force Microscopy (STFM)
Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM)...
PublikationenDiffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS
Diffusion and interaction dynamics of molecules at the plasma membrane play an important role in cellular signaling and are suggested to be strongly associated with the actin cytoskeleton. Here we use...
PublikationenWide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching
Subdiffraction fluorescence imaging is presented in a parallelized wide-field arrangement exploiting the principle of reversible saturable/switchable optical transitions (RESOLFT). The diffraction barrier...
PublikationenReversible photoswitching enables single-molecule fluorescence fluctuation Spectroscopy at high molecular concentration
We demonstrate that photoswitchable markers enable fluorescence fluctuation spectroscopy at high molecular concentration. Reversible photoswitching allows precise control of the density of fluorescing...
PublikationenMonitoring Triplet State Dynamics With Fluorescence Correlation Spectroscopy: Bias and Correction
A marker's dark triplet state is of great importance in fluorescence microscopy: It serves as a means to switch off fluorescent markers and is thus the enabling element for several super-resolution methods....
PublikationenA straightforward approach for gated STED-FCS to investigate lipid membrane dynamics
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these approaches...
PublikationenSuper-resolution STED microscopy advances with yellow CW OPSL
Researchers have a growing need to push optical microscopy beyond the diffraction limit to answer key questions in biology, and stimulated emission depletion (STED) has proven to be a fluorescence imaging...
PublikationenOrientational and dynamical heterogeneity of rhodamine 6G terminally attached to a DNA helix revealed by NMR and single-molecule fluorescence spectroscopy
The comparison of Forster resonance energy transfer (FRET) efficiencies between two fluorophores covalently attached to a single protein or DNA molecule is an elegant approach for deducing information...
PublikationenConvergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS
Fluorescence correlation spectroscopy (FCS) in combination with the super-resolution imaging method STED (STED-FCS), and single-particle tracking (SPT) are able to directly probe the lateral dynamics of...
PublikationenDissecting the actin cortex density and membrane-cortex distance in living cells by super-resolution microscopy
Nanoscale spacing between the plasma membrane and the underlying cortical actin cytoskeleton profoundly modulates cellular morphology, mechanics, and function. Measuring this distance has been a key challenge...
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