6814 Suchergebnisse für: „“
STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact...
PublikationenPeroxisomal Import Reduces the Proapoptotic Activity of Deubiquitinating Enzyme USP2
The human deubiquitinating enzyme ubiquitin-specific protease 2 (USP2) regulates multiple cellular pathways, including cell proliferation and apoptosis. As a result of alternative splicing four USP2 isoenzymes...
PublikationenFluorescence fluctuation spectroscopy in subdiffraction focal volumes
We establish fluorescence fluctuation spectroscopy (FFS) with nanoscale detection volumes generated by stimulated emission depletion. Our method applies fluorescence correlation spectroscopy and fluorescence...
PublikationenBreaking the diffraction barrier in fluorescence microscopy by optical shelving
We report the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by transiently shelving the fluorophore in a metastable dark state. Using a relatively modest light intensity...
PublikationenTriplet-relaxation microscopy with bunched pulsed excitation
Obtaining high signal levels in fluorescence microscopy is usually spoiled by the concomitant population of the dark ( triplet) state of the marker, which is often followed by photobleaching. Recently,...
PublikationenPhotoswitchable Spiropyran Dyads for Biological Imaging
The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photo chromic spironaphthothiopyran unit, which serves as a photoswitchable...
PublikationenNanoscale separation of molecular species based on their rotational mobility
We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution,...
PublikationenAnalytical description of STED microscopy performance
Stimulated emission depletion (STED) resolves fluorescent features that are closer than the far-field optical diffraction limit by applying a spatially modulated light field keeping all but one of these...
PublikationenFluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)
We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion...
PublikationenSTED with wavelengths closer to the emission maximum
In stimulated emission depletion (STED) nanoscopy the wavelength of the STED beam is usually tuned towards the red tail of the emission maximum of the fluorophore. Shifting the STED wavelength closer to...
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