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HDL particles incorporate into lipid bilayers – a combined AFM and single molecule fluorescence microscopy study
The process, how lipids are removed from the circulation and transferred from high density lipoprotein (HDL) - a main carrier of cholesterol in the blood stream - to cells, is highly complex. HDL particles...
PublikationenCytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation
T cell activation and especially trafficking of T cell receptor microclusters during immunological synapse formation are widely thought to rely on cytoskeletal remodeling. However, important details on...
PublikationenAnatomy and dynamics of a supramolecular membrane protein cluster
Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy,...
PublikationenLens-based fluorescence nanoscopy
The majority of studies of the living cell rely on capturing images using fluorescence microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution in the optical...
PublikationenMonitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy
A recently developed, real-time spectroscopic technique, burst-integrated fluorescence lifetime (BIFL), is shown to be well suited for monitoring the individual molecular conformational dynamics of a single...
PublikationenStructure and mechanism of the reversible photoswitch of a fluorescent protein
Proteins that can be reversibly photoswitched between a fluorescent and a nonfluorescent state bear enormous potential in diverse fields, such as data storage, in vivo protein tracking, and subdiffraction...
PublikationenBreaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier has been broken by a saturated...
PublikationenMacromolecular-scale resolution in biological fluorescence microscopy
We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has...
PublikationenStructural basis for reversible photoswitching in Dronpa
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation...
PublikationenFast molecular tracking maps nanoscale dynamics of plasma membrane lipids
We describe an optical method capable of tracking a single fluorescent molecule with a flexible choice of high spatial accuracy (similar to 10-20 nm standard deviation or similar to 20-40 nm full-width-at-half-maximum)...
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