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Single-molecule detection of coumarin-120
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched...
PublikationenExploring single-molecule dynamics with fluorescence nanoscopy
The study of molecular dynamics at the single-molecule level with fluorescence correlation spectroscopy (FCS) and far-field optics has contributed greatly to the functional understanding of complex systems....
PublikationenDark state photophysics of nitrogen-vacancy centres in diamond
Nitrogen-vacancy (NV) colour centres in diamond are attractive fluorescence emitters owing to their unprecedented photostability and superior applicability to spin manipulation and sub-diffraction far-field...
PublikationenNanoscopy of Living Brain Slices with Low Light Levels
Lens-based fluorescence microscopy, which has long been limited in resolution to about 200 nanometers by diffraction, is rapidly evolving into a nanoscale imaging technique. Here, we show that the superresolution...
PublikationenPhosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment
Synaptic-vesicle exocytosis is mediated by the vesicular Ca2+ sensor synaptotagmin-1. Synaptotagmin-1 interacts with the SNARE protein syntaxin-1A and acidic phospholipids such as phosphatidylinositol...
PublikationenThe mystery of membrane organization: composition, regulation and roles of lipid rafts
Cellular plasma membranes are laterally heterogeneous, featuring a variety of distinct subcompartments that differ in their biophysical properties and composition. A large number of studies have focused...
PublikationenDissection of mechanical force in living cells by super-resolved traction force microscopy
Cells continuously exert or respond to mechanical force. Measurement of these nanoscale forces is a major challenge in cell biology; yet such measurement is essential to the understanding of cell regulation...
PublikationenSTED microscopy reveals crystal colour centres with nanometric resolution
Because they have spin states that can be optically polarized and detected, fluorescent nitrogen vacancies in diamond(1-3) have considerable potential for applications in quantum cryptography(4,5) and...
PublikationenMajor signal increase in fluorescence microscopy through dark-state relaxation
We report a substantial signal gain in fluorescence microscopy by ensuring that transient molecular dark states with lifetimes > 1 mu s, such as the triplet state relax between two molecular absorption...
PublikationenFluorescence nanoscopy by ground-state depletion and single-molecule return
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left...
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