6814 Suchergebnisse für: „“
GPU accelerated toolbox for real-time beam-shaping in multimode fibres
We present a GPU accelerated toolbox for shaping the light propagation through multimode fibre using a spatial light modulator (SLM). The light is modulated before being coupled to the proximal end of...
PublikationenHigh-speed spatial control of the intensity, phase and polarisation of vector beams using a digital micro-mirror device
The dynamic spatial control of light fields is essential to a range of applications, from microscopy to optical micro-manipulation and communications. Here we describe the use of a single digital micro-mirror...
PublikationenCompact multimode fiber beam-shaping system based on GPU accelerated digital holography
Real-time, on-demand, beam shaping at the end of the multimode fiber has recently been made possible by exploiting the computational power of rapidly evolving graphics processing unit (GPU) technology...
PublikationenOptical sorting and detection of submicrometer objects in a motional standing wave
An extended interference pattern close to the surface may result in either a transmissive or an evanescent surface field for large-area manipulation of trapped particles. The affinity of differing particle...
PublikationenLong-range one-dimensional longitudinal optical binding
We create extended longitudinally optically bound chains of microparticles with the use of counter-propagating "nondiffracting'' light fields, the so-called Bessel beams. The beam homogeneity and extended...
PublikationenFemtosecond Optoinjection of Intact Tobacco BY-2 Cells Using a Reconfigurable Photoporation Platform
A tightly-focused ultrashort pulsed laser beam incident upon a cell membrane has previously been shown to transiently increase cell membrane permeability while maintaining the viability of the cell, a...
PublikationenMultimode fibre: Light-sheet microscopy at the tip of a needle
Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue....
PublikationenUse of BODIPY-Cholesterol (TF-Chol) for Visualizing Lysosomal Cholesterol Accumulation
Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol...
PublikationenCortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane – a minimally invasive investigation by STED-FCS
Important discoveries in the last decades have changed our view of the plasma membrane organisation. Specifically, the cortical cytoskeleton has emerged as a key modulator of the lateral diffusion of membrane...
PublikationenCalQuo: automated, simultaneous single-cell and population-level quantification of global intracellular Ca2+ responses
Detecting intracellular calcium signaling with fluorescent calcium indicator dyes is often coupled with microscopy techniques to follow the activation state of non-excitable cells, including lymphocytes....
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