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Sharper low-power STED nanoscopy by time gating
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details...
PublikationenNanoscopy with more than 100,000 ‚doughnuts‘
We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently...
PublikationenMulti-protein assemblies underlie the mesoscale organization of the plasma membrane
Most proteins have uneven distributions in the plasma membrane. Broadly speaking, this may be caused by mechanisms specific to each protein, or may be a consequence of a general pattern that affects the...
PublikationenScanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells
The interaction of lipids and proteins plays an important role in plasma membrane bioactivity, and much can be learned from their diffusion characteristics. Here we present the combination of super-resolution...
PublikationenHydrophobic mismatch sorts SNARE proteins into distinct membrane domains
The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning of biological membranes. Such domain formation can be the result of hydrophobic...
PublikationenA simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization
Intramolecular photostabilization via triple-state quenching was recently revived as a tool to impart synthetic organic fluorophores with 'self-healing' properties. To date, utilization of such fluorophore...
PublikationenSelf-organizing actin patterns shape membrane architecture but not cell mechanics
Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic...
PublikationenA dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome
Many bacteria use a type III secretion system (T3SS) to inject effector proteins into host cells. Selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface...
PublikationenEnvelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state
Human immunodeficiency virus type 1 (HIV-1) assembles as immature particles, which require the proteolytic cleavage of structural polyprotein Gag and the clustering of envelope glycoprotein Env for infectivity....
PublikationenA near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine...
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