6814 Suchergebnisse für: „“

Single-step purification of T7 RNA polymerase with a 6-histidine tag

Complex patterns predicted in an in vitro experimental model system for the evolution of molecular cooperation

An isothermal biochemical in vitro amplification system with two trans-cooperatively coupled amplifying DNA molecules was investigated homogeneously using a hierarchy of kinetic models and as a simplified...

Residual DNA in thermostable DNA polymerases – a cause of irritation in diagnostic PCR and microarray assays

In a validation trial of a DNA microarray test for chlamydiae we repeatedly observed false-positive PCR amplicons from truly negative samples and non-template controls. Various PCR tests, microarray hybridization...

Purification and properties of L(-)-carnitine dehydrogenase from Agrobacterium sp.

l(−)-Carnitine:NAD+ oxidoreductase, EC 1.1.1.108, from Agrobacterium sp. catalyzes the oxidation of l(−)-carnitine to 3-dehydrocarnitine as initial step of l(−)-carnitine degradation. The enzyme...

Phänotypisierung und Microarraybasierte Genotypisierung des Antibiotika-Resistenzverhaltens von MRSA und MSSA aus Viertelgemelksproben klinisch gesunder Milchkühe [Phenotyping and microarray based genotyping of the antibiotic resistance of MRSA and MSSA from quarter milk samples of clinically healthy dairy cows]

Ziel dieser Untersuchung war es, in einer systematischen Stichprobe von Staphy- lococcus aureus-Isolaten aus Rohmilch klinisch gesunder Kühe eine umfassende Charakterisierung des Antibiotika-Resistenzverhaltens...

Zoonotic Agents in Small Ruminants Kept on City Farms in Southern Germany

Sheep and goats are popular examples of livestock kept on city farms. In these settings, close contacts between humans and animals frequently occur. Although it is widely accepted that small ruminants...

Genotypic and Phenotypic Characterization of Staphylococcus aureus Isolates from Wild Boars

Eight Staphylococcus aureus isolates collected from 117 wild boars were characterized and compared to livestock isolates. They belonged to sequence types ST133, ST425, and the new type ST1643. The spa...

Presence of Enterohemorrhagic Escherichia coli ST678/O104:H4 in France Prior to 2011

Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and...

Rapid Microarray-Based Genotyping of Enterohemorrhagic Escherichia coli Serotype O156:H25/H-/Hnt Isolates from Cattle and Clonal Relationship Analysis

Since enterohemorrhagic Escherichia coli (EHEC) isolates of serogroup O156 have been obtained from human diarrhea patients and asymptomatic carriers, we studied cattle as a potential reservoir for these...

Pathotyping Escherichia coli by using miniaturized DNA microarrays

The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray...