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Correlative spectral STED nanoscopy and STED-FCS elucidate the origin of anomalous diffusion in cellular membranes
Advances in our understanding of the nanoscale architecture of the plasma membrane heavily rely on the development of non-invasive experimental methods, particularly of advanced fluorescence microscopy...
Publikationen | 25.10.2023Tracking membrane dynamics on stem cell-derived neurons using 3D MINFLUX
Actin rings are periodic cytoskeletal structures found in dendrites and neuronal cells that regulate the shape and stability of dendritic spines. They are also known to act as a semi-permeable barrier...
Publikationen | 25.10.2023How to use advanced microscopy to study peroxisomes – a multimodal approach from FCS, photoactivation and STED to Minflux
Peroxisomes are small (100-300 nm) intracellular organelles that fulfil many anabolic and catabolic functions. Mistargeting of peroxisomal proteins, which are imported from the cytosol, therefore leads...
Publikationen | 25.10.2023Biomolecular condensates in contact with membranes: interaction mechanism, wetting, and complex remodeling
Membrane wetting by biomolecular condensates recently emerged as an important phenomenon in cell biology. It plays a vital role in a diverse range of processes across different organisms. However, the...
Publikationen | 25.10.2023Measuring lipid dynamics and packing with MINFLUX microscopy
The eukaryotic cell plasma membrane is highly complex, and its organization is critical to multiple biological processes, such as virus infection. Still, the role of membrane heterogeneity remains poorly...
Publikationen | 25.10.2023Dynamic single particle measurements with a high throughput iSCAT-TIRF setup
Interferometric Scattering Microscopy (iSCAT) enables label free, prolonged and high frame rate image acquisition. Typically, iScat setups employ scanning illumination schemes to achieve uniform sample...
Publikationen | 24.10.2023Avoiding photoblueing-induced artefacts in confocal and STED microscopy
Confocal and stimulated emission depletion (STED) superresolution microscopy are scanning microscopy techniques widely used in life sciences. Unfortunately, commonly used laser excitation powers can induce...
Publikationen | 24.10.2023napari-live-recording: embedding camera acquisition in napari
Custom-built microscopes often require to control hardware objects directly. A high-complexity device group is cameras. Moreover, camera producers provide softwares which often lack the features needed...
Publikationen | 24.10.2023AI enables nanotextural multiplexing of sub-cellular structures
With the rise of optical super-resolution microscopy (SRM), intracellular structures can be analyzed with nanometer resolution. Here, specific fluorescent markers enable multi-color imaging of several...
Publikationen | 24.10.2023Decoding the scanning parameters of iterative MINFLUX through Single Particle Tracking
Detecting the lateral dynamics of molecules on living and model membranes is a challenging field of study, requiring microscopy techniques that can track molecules with single particle level of detail,...
Publikationen | 24.10.2023