Objective. Since enamel is not capable of regeneration and dentin repair is dependent on odontoblasts and is directed towards the pulp, current treatment options aim to seal or fill these lesions. Previous research has demonstrated that biomimetic mineralization can result in an enamel-like layer containing fluorapatite. The purpose of this study was to investigate the effect of an experimental biomimetic mineralization kit (BIMIN) on the chemical composition and crystallinity of enamel and dentin in-vitro. Methods. Enamel and dentin samples from 20 human teeth (10 for enamel; 10 for dentin) were divided into a control group without treatment and test samples with BIMIN treatment. Quantitative analysis of tissue penetration of fluoride, phosphate, and calcium was performed using energy-dispersive X-ray spectroscopy (EDX). Mineralization depth was measured with a Raman spectrometer (spectral range from 900 to 1020 cm-1). EDX data was statistically analyzed using a paired t-test and Raman data was analyzed using the Student’s t-test. Results. EDX analysis demonstrated a penetration depth of fluoride of 4.10 ± 3.32 µm in enamel and 4.31 ± 2.67 µm in dentin. Calcium infiltrated into enamel 2.65 ± 0.64 µm and into dentin 5.58 ± 1.63 µm, while the penetration depths for phosphate were 4.83 ± 2.81 µm for enamel and 6.75 ± 3.25 µm for dentin. Further, up to 25 µm of a newly mineralized enamel-like layer was observed on the surface of the samples. Raman concentration curves demonstrated an increased degree of mineralization up to 5 -10 µm into the dentin and enamel samples. Significance. Biomimetic mineralization of enamel and dentin samples resulted in newly formed enamel-like layers on both enamel and dentin. There appears to be deep bonding between the BIMIN-layer and the tooth structure with an increase of mineralization and a penetration of fluoride into enamel and dentin.