A specific spectral signature of serum and plasma-derived extracellular vesicles for cancer screening

Infrared and Raman spectroscopy were used for comprehensive comparative analysis of cancer versus non-cancer extracellular vesicles (EV). Serum and plasma EV were subjected to drop-coating deposition and drying on calcium fluoride substrates. We determined enhancement of beta-sheet-rich proteins as a cancer-specific blood EV signature and successfully applied it for detection of prostate cancer in a test cohort of patients with high-grade prostate carcinoma and benign hypoplasia.