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Raman spectroscopic investigation of the human liver stem cell line HepaRG

Ryabchykov, Oleg; Bräutigam, Katharina; Galler, Kerstin; Neugebauer, Ute; Mosig, Alexander; Bocklitz, Thomas W.; Popp, Jürgen
in: Journal of Raman Spectroscopy (2018) 935

In this work, Raman spectroscopic cell imaging approaches and a discrimination between HepG2, non-differentiated HepaRG, and differentiated hepatocyte-like HepaRG cells are presented. Raman spectroscopic imaging was used to visualize the cell nuclei by means of false color imaging of a marker band and a cell segmentation was performed by means of clustering. Furthermore, a 3-class-classification model based on the mean Raman spectra of individual cells was established for a classification between different cell types. A high average sensitivity of 96% was achieved by the applied classification model. Based on the results of clustering and classification, the main spectral contributions to different cell types and cell segments were analyzed in detail. Thereby, HepG2, non-differentiated HepaRG, and differentiated hepatocyte-like HepaRG cells were Raman spectroscopic characterized and proven to be significantly different.

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