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Loop mediated amplification as promising on-site detection approach for Legionella pneumophila and Legionella spp.

Reuter, Cornelia; Slesiona, Nicole; Hentschel, Stefanie; Aehlig, Oliver; Breitenstein, Antje; Csáki, Andrea; Fritzsche, Wolfgang
in: Applied Microbiology and Biotechnology (2020) 405

Recently Legionella pneumophila is the main causative waterborne organism of severe respiratory infections. Additionally, other Legionella species are documented as human pathogens. In our work we describe a rapid detection method which combines two advantages for sensitive and specific detection of the genus Legionella: the fast isothermal amplification method “Loop-mediated isothermal AMPlification” (LAMP), and a colorimetric detection method using the metal indicator hydroxynaphtol blue (HBN) which allows to determine an optical signal with a simple read out (with the naked eye). Moreover, we present two approaches for minimizing the assay volume using a stationary microchip LAMP and droplet digital based LAMP (ddLAMP) as promising high sensitive setups.

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