The aim of the project is to establish a bioanalytical platform for the detection of important bovine pathogens. In this context, pathogen DNA is detected as a biomarker by means of an innovative detection technique (loop mediated isothermal amplification LAMP), which, in contrast to conventional molecular biological methods such as PCR, can be performed with simple instrumentation and thus on-site. While the German partner is developing and realizing a suitable robust, compact and cost-effective instrument both for the performance of LAMP (requires 60°C) and for the subsequent colorimetric detection (by means of LED spectrophotometer), the Cuban partner is developing the corresponding LAMP assays for the pathogens A. marginal and B. marginal. pathogens A. marginale and B. bovis will be established by the Cuban partner and subsequently transferred to the integrated instrument where they will be tested and validated. In parallel, training of the Cuban operators will take place. The platform is open and can be extended on the part of the Cuban partner for further LAMP-based assays, likewise a manufacturing of this device in Cuba is conceivable. The project will establish a permanent relationship between the two working groups. Besides the direct project work, a joint workshop, involvement in teaching activities (seminars) and the integration of the partners into local networks are planned. In particular, young scientists will be involved.

This project is funded by the German Ferderal Ministry of Education and Research (BMBF) and the German Aerospace Center (DLR). 

Project partners
Group of Bacteriology-Parasitology, Direction of Animal Health
National Center for Animal and Plant Health (CENSA)
San José de las Lajas, Mayabeque, Cuba