Single-molecule detection of coumarin-120

in: Nucleosides & Nucleotides (1997)
Brand, L.; Eggeling, Christian; Seidel, Claus A.M.
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resulting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasenstive DNA characterization.

Third party cookies & scripts

This site uses cookies. For optimal performance, smooth social media and promotional use, it is recommended that you agree to third party cookies and scripts. This may involve sharing information about your use of the third-party social media, advertising and analytics website.
For more information, see privacy policy and imprint.
Which cookies & scripts and the associated processing of your personal data do you agree with?

You can change your preferences anytime by visiting privacy policy.