Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies

in: Molecular and Cellular Probes (2006)
Slickers, Peter; Goellner, Stefanie; Hotzel, Helmut; Sachse, Konrad; Ehricht, Ralf
This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples.

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