Polarized illumination coded structured illumination microscopy (picoSIM): experimental results

in: Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences (2022)
Appelt, Daniel; Ehler, Elisabeth; Heintzmann, Rainer; Wicker, Kai; Shukla Mukherjee, Sapna
The need for acquiring at least three images to reconstruct an optical section of a sample limits the acquisition rate in structured illumination microscopy (SIM) for optical sectioning. In polarized illumination coded structured illumination microscopy (picoSIM) the three individual light patterns are encoded in a single polarized illumination light distribution, enabling the acquisition of the complete SIM data in a single exposure. Here, we describe our experimental set-up and show experimental results acquired with sequential and single-shot picoSIM. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’.

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